Of all the %&$%!!

Arexack_Heretic · Post by **Arexack_Heretic** » Thu Jul 05, 2007 8:07 am

Aargh!

Someone turned off my incubator yesterday,
probably killing my precious bacteria.

Hopefully they will still grow before the day is up, otherwise I'll have to reinoculate them and that will set me back more than just a single day, because there is no way I'm going to have time to come back to the lab on saturday and....AAARGH!
Grumble grrr!

[/end rant]

Selezen · Post by **Selezen** » Thu Jul 05, 2007 9:18 am

That't the winner for "Most Bizarre Post" for this year.

Arexack_Heretic · Post by **Arexack_Heretic** » Thu Jul 05, 2007 1:29 pm

hmm?
nobody posts his outrage at the world in Outworld?

Commander McLane · Post by **Commander McLane** » Fri Jul 06, 2007 11:28 pm

Perhaps in a slightly more understandable way?

Anyway: I try to guess that you are sort of in the biology business and a sort of experiment was sort of failing because of what sort of happened.

If I got that sort of right, then you have sort of my deepest condolences!

Captain Hesperus · Post by **Captain Hesperus** » Sat Jul 07, 2007 10:45 pm

Commander McLane wrote:
Anyway: I try to guess that you are sort of in the biology business and a sort of experiment was sort of failing because of what sort of happened.

No, A_H is attempting to genetically engineer Trumbles using bacterial DNA and RNA strands to properly replicate the Trumble's rapid multiplication.

Captain Hesperus

TGHC · Post by **TGHC** » Sat Jul 07, 2007 10:46 pm

Arexack_Heretic · Post by **Arexack_Heretic** » Mon Jul 09, 2007 9:38 am

indeed I am using trumbles as a vector to replicate my DNA samples in a rapid manner

Wiggy · Post by **Wiggy** » Mon Jul 09, 2007 3:16 pm

Arexack_Heretic wrote:
Someone turned off my incubator yesterday,
probably killing my precious bacteria.

Perhaps you could send me some of these difficult-to-cultivate bacteria?
In my bathroom, I only have the easy-grow variety. (Gram negative, of course.)

Arexack_Heretic · Post by **Arexack_Heretic** » Tue Jul 10, 2007 9:18 am

they may insert their cargo into your DNA though. These agrobacteriums are used as vectors to insert genes into plants, but may do so in mammals too on occasion.

Managed to grow them up during the day, then did a large culture overnight and the day after.
Then I messed up myself and took the wrong plantlines to transform with them.

I'll have to grow a whole new batch of target plants from heterozygous seed now.
(I need the heterozygous plants, but as Arabidopsis selfcrosses to form seed, the seed I get is 1/2 heterozygous, 1/4 homozygous mutant and 1/4 wildtype.)

This will take approximately 3-4 weeks.

Code: Select all

I sterilized seeds yesterday and these are now on an agarplate at 4degrees to be vernalised (cold activation) 5days.
grow to seedling 1 week. 
remove deliterious triple homozygous plants.
genotype by PCR for heterozygous plants.
pot heterouzygous in soil.
grow to bolting stage(2-3 weeks), trim, grow several more days.
(meanwhile regrow agrobacterium cultures.)
redo transformations.

sigh.

Star Gazer · Post by **Star Gazer** » Tue Jul 10, 2007 2:42 pm

Arexack_Heretic wrote:
they may insert their cargo into your DNA though. ...

oooo... ...young man...

You'd have got on well with Mad Dan Eccles. He may have even understood what you said...

Arexack_Heretic · Post by **Arexack_Heretic** » Tue Jul 10, 2007 3:21 pm

I think I met him a few times here.

One of those things that make me real tired:

I used a coworker's growth medium and i inadvertantly contaminated it.
Being the good guy that is me, I set out to replace the medium and make some for myself as well.
All is well, it is a simple medium, but still I have to weigh 6 seperate components. I dissolve them in distilled water.
-at this point I decide to work on the genotyping I was ranting about earlier-
I come back to the medium and take a squeezebottle of MQ to adjust the solution to its final volume.
The liquid I am discharging in the medium makes nice swirly patterns and makes the stuff go turbid.
"WHAT THE %$@#@!!!", I think, this is not supposed to happen.
I smell the yeasty crap that is now in the bottle, it smells of bleach.
I make some unintelligable rumbling noises and the Brazillian pHD student looks at me in a funny way. I ask him why the bottle marked MQ contains a 50% bleach solution. He says: "Dunno". Apparently I can not kick him as he is obviously innocent of the crime.
With a sigh and mentioning this kinda crap makes me unhappy I upturn the bottle in the sink.
I repeat the procedure this time making sure water is indeed just that.
...
At this time, I realise I did not rinse the bottle with water and will probably contain trace amounts of bleach still.

Frame · Post by **Frame** » Tue Jul 10, 2007 3:45 pm

Arexack_Heretic wrote:
I think I met him a few times here.

One of those things that make me real tired:

I used a coworker's growth medium and i inadvertantly contaminated it.
Being the good guy that is me, I set out to replace the medium and make some for myself as well.
All is well, it is a simple medium, but still I have to weigh 6 seperate components. I dissolve them in distilled water.
-at this point I decide to work on the genotyping I was ranting about earlier-
I come back to the medium and take a squeezebottle of MQ to adjust the solution to its final volume.
The liquid I am discharging in the medium makes nice swirly patterns and makes the stuff go turbid.
"WHAT THE %$@#@!!!", I think, this is not supposed to happen.
I smell the yeasty crap that is now in the bottle, it smells of bleach.
I make some unintelligable rumbling noises and the Brazillian pHD student looks at me in a funny way. I ask him why the bottle marked MQ contains a 50% bleach solution. He says: "Dunno". Apparently I can not kick him as he is obviously innocent of the crime.
With a sigh and mentioning this kinda crap makes me unhappy I upturn the bottle in the sink.
I repeat the procedure this time making sure water is indeed just that.
...
At this time, I realise I did not rinse the bottle with water and will probably contain trace amounts of bleach still.

Suddenly i understand so much.....

Arexack_Heretic · Post by **Arexack_Heretic** » Tue Jul 10, 2007 3:50 pm

Suddenly i understand so much.....

Success!
Finally I can share my dismay at failure with one who understands my pain.
Much relief and feelings of grief-shared.

Makandal · Post by **Makandal** » Wed Jul 11, 2007 8:22 am

I was studying oenology (And I abandonned, this is NOT tasting wine at all, there is only 20 h of wine tasting in one year) and had microbiology and biochemistry as well. This was one of the most boring of all. I remembered to have spit in a bottle of starch to accelerate the transformation in sugar... All the results were quite odd !
By the way, are Thargoids spaceships semi organics ? I always thought they were grown organically and made of something like chitins.

Arexack_Heretic · Post by **Arexack_Heretic** » Wed Jul 11, 2007 9:15 am

Could be.

Personally I am fed up with the bad-guys always having organic ships.

My prefered option is that Thargoids have a racial tendency towards octagonal designs similar to humans building everything to square angles.

hexagonal or triangular tendency would be more logical for a spaceborne insect, but hell this is a fantasy ooniverse.

The sketches provided in the elite manual support this idea as they show a blockish design, not biologically curving lines.
(a thargoid could look like a barnackle or a limpet or maybe a seastar if you want to do organic)

.....

It should be fairly easy to model a macro-bacterium modeled on e.coli.
Want to be rammed by a large flaggellum propelled bacterium?

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